NAME

SYNOPSIS

DESCRIPTION

Either the query queryfile or the target seqdb may be '-' (a dash character), in which case the query file or target database input will be read from a <stdin> pipe instead of from a file. Only one input source can come through <stdin>, not both. If the queryfile contains more than one query, then seqdb cannot come from stdin, because we can't rewind the streaming target database to search it with another profile.

If the query is sequence-based, a new file containing the HMM(s) built from the input(s) in queryfile may optionally be produced, with the filename set using the --hmmout flag.

The output format is designed to be human-readable, but is often so voluminous that reading it is impractical, and parsing it is a pain. The --tblout option saves output in a simple tabular format that is concise and easier to parse. The -o option allows redirecting the main output, including throwing it away in /dev/null.

OPTIONS

-h

Help; print a brief reminder of command line usage and all available options.

OPTIONS FOR CONTROLLING OUTPUT

-o <f>

Direct the main human-readable output to a file <f> instead of the default stdout.

-A <f>

Save a multiple alignment of all significant hits (those satisfying "inclusion thresholds") to the file <f>.

--tblout <f>

Save a simple tabular (space-delimited) file summarizing the per-target output, with one data line per homologous target sequence found.

--dfamtblout <f>

Save a tabular (space-delimited) file summarizing the per-hit output, similar to --tblout but more succinct.

--aliscoresout <f>

Save to file a list of per-position scores for each hit. This is useful, for example, in identifying regions of high score density for use in resolving overlapping hits from different models.

--hmmout <f>

If queryfile is sequence-based, write the internally-computed HMM(s) to file <f>.

--acc

Use accessions instead of names in the main output, where available for profiles and/or sequences.

--noali

Omit the alignment section from the main output. This can greatly reduce the output volume.

--notextw

Unlimit the length of each line in the main output. The default is a limit of 120 characters per line, which helps in displaying the output cleanly on terminals and in editors, but can truncate target profile description lines.

--textw <n>

Set the main output's line length limit to <n> characters per line. The default is 120.

OPTIONS CONTROLLING SINGLE SEQUENCE SCORING

--singlemx

If a single sequence query comes from a multiple sequence alignment file, such as in Stockholm format, the search model is by default constructed as is typically done for multiple sequence alignments. This option forces nhmmer to use the single-sequence method with substitution score matrix.

--mxfile<mxfile

Obtain residue alignment probabilities from the substitution matrix in file mxfile. The default score matrix is DNA1 (this matrix is internal to HMMER and does not have to be available as a file). The format of a substitution matrix mxfile is the standard format accepted by BLAST, FASTA, and other sequence analysis software. See ftp.ncbi.nlm.nih.gov/blast/matrices/ for example files. (The only exception: we require matrices to be square, so for DNA, use files like NCBI's NUC.4.4, not NUC.4.2.)

--popen <x>

Set the gap open probability for a single sequence query model to <x>. The default is 0.02. <x> must be >= 0 and < 0.5.

--pextend <x>

Set the gap extend probability for a single sequence query model to <x>. The default is 0.4. <x> must be >= 0 and < 1.0.

OPTIONS CONTROLLING REPORTING THRESHOLDS

-E <x>

Report target sequences with an E-value of <= <x>. The default is 10.0, meaning that on average, about 10 false positives will be reported per query, so you can see the top of the noise and decide for yourself if it's really noise.

-T <x>

Instead of thresholding output on E-value, instead report target sequences with a bit score of >= <x>.

OPTIONS FOR INCLUSION THRESHOLDS

--incE <x>

Use an E-value of <= <x> as the inclusion threshold. The default is 0.01, meaning that on average, about 1 false positive would be expected in every 100 searches with different query sequences.

--incT <x>

Instead of using E-values for setting the inclusion threshold, use a bit score of >= <x> as the inclusion threshold. By default this option is unset.

OPTIONS FOR MODEL-SPECIFIC SCORE THRESHOLDING

To use these options, the profile must contain the appropriate (GA, TC, and/or NC) optional score threshold annotation; this is picked up by hmmbuild from Stockholm format alignment files. For a nucleotide model, each thresholding option has a single per-hit threshold <x> This acts as if -T <x> --incT <x> has been applied specifically using each model's curated thresholds.

--cut_ga

Use the GA (gathering) bit score threshold in the model to set per-hit reporting and inclusion thresholds. GA thresholds are generally considered to be the reliable curated thresholds defining family membership; for example, in Dfam, these thresholds are applied when annotating a genome with a model of a family known to be found in that organism. They may allow for minimal expected false discovery rate.

--cut_nc

Use the NC (noise cutoff) bit score threshold in the model to set per-hit reporting and inclusion thresholds. NC thresholds are less stringent than GA; in the context of Pfam, they are generally used to store the score of the highest-scoring known false positive.

--cut_tc

Use the TC (trusted cutoff) bit score threshold in the model to set per-hit reporting and inclusion thresholds. TC thresholds are more stringent than GA, and are generally considered to be the score of the lowest-scoring known true positive that is above all known false positives; for example, in Dfam, these thresholds are applied when annotating a genome with a model of a family not known to be found in that organism.

OPTIONS CONTROLLING THE ACCELERATION PIPELINE

Changing filter thresholds only removes or includes targets from consideration; changing filter thresholds does not alter bit scores, E-values, or alignments, all of which are determined solely in postprocessing.

--max

Turn off (nearly) all filters, including the bias filter, and run full Forward/Backward postprocessing on most of the target sequence. In contrast to phmmer and hmmsearch, where this flag really does turn off the filters entirely, the --max flag in nhmmer sets the scanning-SSV filter threshold to 0.4, not 1.0. Use of this flag increases sensitivity somewhat, at a large cost in speed.

--F1 <x>

Set the P-value threshold for the SSV filter step. The default is 0.02, meaning that roughly 2% of the highest scoring nonhomologous targets are expected to pass the filter.

--F2 <x>

Set the P-value threshold for the Viterbi filter step. The default is 0.001.

--F3 <x>

Set the P-value threshold for the Forward filter step. The default is 1e-5.

--nobias

Turn off the bias filter. This increases sensitivity somewhat, but can come at a high cost in speed, especially if the query has biased residue composition (such as a repetitive sequence region, or if it is a membrane protein with large regions of hydrophobicity). Without the bias filter, too many sequences may pass the filter with biased queries, leading to slower than expected performance as the computationally intensive Forward/Backward algorithms shoulder an abnormally heavy load.

OPTIONS FOR SPECIFYING THE ALPHABET

--amino

Assert that sequences in msafile are protein, bypassing alphabet autodetection.

--dna

Assert that sequences in msafile are DNA, bypassing alphabet autodetection.

--rna

Assert that sequences in msafile are RNA, bypassing alphabet autodetection.

OPTIONS CONTROLLING SEED SEARCH HEURISTIC

Changing parameters for this seed-finding step will impact both speed and sensitivity - typically faster search leads to lower sensitivity.

--seed_max_depth <n>

The seed step requires that a seed reach a specified bit score in length no longer than <n>. By default, this value is 15. Longer seeds allow a greater chance of meeting the bit score threshold, leading to diminished filtering (greater sensitivity, slower run time).

--seed_sc_thresh <x>

The seed must reach score <x> (in bits). The default is 15.0 bits. A higher threshold increases filtering stringency, leading to faster run times and lower sensitivity.

--seed_sc_density <x>

Either all prefixes or all suffixes of a seed must have bit density (bits per aligned position) of at least <x>. The default is 0.8 bits/position. An increase in the density requirement leads to increased filtering stringency, thus faster run times and lower sensitivity.

--seed_drop_max_len <n>

A seed may not have a run of length <n> in which the score drops by --seed_drop_lim or more. Basically, this prunes seeds that go through long slightly-negative seed extensions. The default is 4. Increasing the limit causes (slightly) diminished filtering efficiency, thus slower run times and higher sensitivity. (minor tuning option)

--seed_drop_lim <x>

In a seed, there may be no run of length --seed_drop_max_len in which the score drops by --seed_drop_lim. The default is 0.3 bits. Larger numbers mean less filtering. (minor tuning option)

--seed_req_pos <n>

A seed must contain a run of at least <n> positive-scoring matches. The default is 5. Larger values mean increased filtering. (minor tuning option)

--seed_ssv_length <n>

After finding a short seed, an ungapped alignment is extended in both directions in an attempt to meet the --F1 score threshold. The window through which this ungapped alignment extends is length <n>. The default is 70. Decreasing this value slightly reduces run time, at a small risk of reduced sensitivity. (minor tuning option)

OTHER OPTIONS

--qhmm

Assert that the input queryfile contains one or more profile HMMs, as built by hmmbuild.

--qfasta

Assert that the input queryfile contains one or more unaligned sequences, stored in fasta format.

--qmsa

Assert that the input queryfile contains one or more sequence alignments. The format of the file may be specified with the --qformat flag.

--qformat <s>

Assert that input queryfile is a sequence file (unaligned or aligned), in format <s>, bypassing format autodetection. Common choices for <s> include: fasta, embl, genbank. Alignment formats also work; common choices include: stockholm, a2m, afa, psiblast, clustal, phylip. For more information, and for codes for some less common formats, see main documentation. The string <s>

--tformat <s>

Assert that target sequence database seqdb is in format <s>, bypassing format autodetection. Common choices for <s> include: fasta, embl, genbank, ncbi, fmindex. Alignment formats also work; common choices include: stockholm, a2m, afa, psiblast, clustal, phylip. For more information, and for codes for some less common formats, see main documentation. The string <s> is case-insensitive (fasta or FASTA both work). The format ncbi indicates that the database file is a binary file produced using makeblastdb. The format fmindex indicates that the database file is a binary file produced using makehmmerdb.

--nonull2

Turn off the null2 score corrections for biased composition.

-Z <x>

For the purposes of per-hit E-value calculations, Assert that the total size of the target database is <x> million nucleotides, rather than the actual number of targets seen.

--seed <n>

Set the random number seed to <n>. Some steps in postprocessing require Monte Carlo simulation. The default is to use a fixed seed (42), so that results are exactly reproducible. Any other positive integer will give different (but also reproducible) results. A choice of 0 uses a randomly chosen seed.

--w_beta <x>

Window length tail mass. The upper bound, W, on the length at which nhmmer expects to find an instance of the model is set such that the fraction of all sequences generated by the model with length >= W is less than <x>. The default is 1e-7. This flag may be used to override the value of W established for the model by hmmbuild, or when the query is sequence-based.

--w_length <n>

Override the model instance length upper bound, W, which is otherwise controlled by --w_beta. It should be larger than the model length. The value of W is used deep in the acceleration pipeline, and modest changes are not expected to impact results (though larger values of W do lead to longer run time). This flag may be used to override the value of W established for the model by hmmbuild, or when the query is sequence-based.

--watson

Only search the top strand. By default both the query sequence and its reverse-complement are searched.

--crick

Only search the bottom (reverse-complement) strand. By default both the query sequence and its reverse-complement are searched.

--cpu <n>

Set the number of parallel worker threads to <n>. On multicore machines, the default is 2. You can also control this number by setting an environment variable, HMMER_NCPU. There is also a master thread, so the actual number of threads that HMMER spawns is <n>+1.

This option is not available if HMMER was compiled with POSIX threads support turned off.

--stall

For debugging the MPI master/worker version: pause after start, to enable the developer to attach debuggers to the running master and worker(s) processes. Send SIGCONT signal to release the pause. (Under gdb: (gdb) signal SIGCONT) (Only available if optional MPI support was enabled at compile-time.)

--mpi

Run under MPI control with master/worker parallelization (using mpirun, for example, or equivalent). Only available if optional MPI support was enabled at compile-time.

SEE ALSO

For complete documentation, see the user guide that came with your HMMER distribution (Userguide.pdf); or see the HMMER web page (http://hmmer.org/).

COPYRIGHT

Copyright (C) 2019 Howard Hughes Medical Institute.
Freely distributed under the BSD open source license.

For additional information on copyright and licensing, see the file called COPYRIGHT in your HMMER source distribution, or see the HMMER web page (http://hmmer.org/).

AUTHOR

http://eddylab.org