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NAMEBio::Cluster::UniGene - UniGene objectSYNOPSISuse Bio::Cluster::UniGene; use Bio::ClusterIO; $stream = Bio::ClusterIO->new('-file' => "Hs.data", '-format' => "unigene"); # note: we quote -format to keep older perl's from complaining. while ( my $in = $stream->next_cluster() ) { print $in->unigene_id() . "\n"; while ( my $sequence = $in->next_seq() ) { print $sequence->accession_number() . "\n"; } } DESCRIPTIONThis UniGene object implements the Bio::Cluster::UniGeneI interface for the representation if UniGene clusters in Bioperl. It is returned by the Bio::ClusterIO parser for unigene format and contains all the data associated with one UniGene record.This class implements several interfaces and hence can be used wherever instances of such interfaces are expected. In particular, the interfaces are Bio::ClusterI as the base interface for all cluster representations, and in addition Bio::IdentifiableI and Bio::DescribableI. The following lists the UniGene specific methods that are available (see below for details). Be aware next_XXX iterators take a snapshot of the array property when they are first called, and this snapshot is not reset until the iterator is exhausted. Hence, once called you need to exhaust the iterator to see any changes that have been made to the property in the meantime. You will usually want to use the non-iterator equivalents and loop over the elements yourself. new() - standard new call unigene_id() - set/get unigene_id title() - set/get title (description) gene() - set/get gene cytoband() - set/get cytoband mgi() - set/get mgi locuslink() - set/get locuslink homol() - set/get homologene gnm_terminus() - set/get gnm_terminus scount() - set/get scount express() - set/get express, currently takes/returns a reference to an array of expressed tissues next_express() - returns the next tissue expression from the expressed tissue array chromosome() - set/get chromosome, currently takes/returns a reference to an array of chromosome lines next_chromosome() - returns the next chromosome line from the array of chromosome lines sts() - set/get sts, currently takes/returns a reference to an array of sts lines next_sts() - returns the next sts line from the array of sts lines txmap() - set/get txmap, currently takes/returns a reference to an array of txmap lines next_txmap() - returns the next txmap line from the array of txmap lines protsim() - set/get protsim, currently takes/returns a reference to an array of protsim lines next_protsim() - returns the next protsim line from the array of protsim lines sequences() - set/get sequence, currently takes/returns a reference to an array of references to seq info next_seq() - returns a Seq object that currently only contains an accession number Implemented InterfacesThis class implementes the following interfaces.
FEEDBACKMailing ListsUser feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated.bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists SupportPlease direct usage questions or support issues to the mailing list:bioperl-l@bioperl.org rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. Reporting BugsReport bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:https://github.com/bioperl/bioperl-live/issues AUTHOR - Andrew MacgregorEmail andrew at cbbc.murdoch.edu.auCONTRIBUTORSHilmar Lapp, hlapp at gmx.netAPPENDIXThe rest of the documentation details each of the object methods. Internal methods are usually preceded with a "_".newTitle : new Usage : used by ClusterIO Returns : a new Bio::Cluster::Unigene object Bio::Cluster::UniGeneI methodsunigene_idTitle : unigene_id Usage : unigene_id(); Function: Returns the unigene_id associated with the object. Example : $id = $unigene->unigene_id or $unigene->unigene_id($id) Returns : A string Args : None or an id titleTitle : title Usage : title(); Function: Returns the title associated with the object. Example : $title = $unigene->title or $unigene->title($title) Returns : A string Args : None or a title geneTitle : gene Usage : gene(); Function: Returns the gene associated with the object. Example : $gene = $unigene->gene or $unigene->gene($gene) Returns : A string Args : None or a gene cytobandTitle : cytoband Usage : cytoband(); Function: Returns the cytoband associated with the object. Example : $cytoband = $unigene->cytoband or $unigene->cytoband($cytoband) Returns : A string Args : None or a cytoband mgiTitle : mgi Usage : mgi(); Function: Returns the mgi associated with the object. Example : $mgi = $unigene->mgi or $unigene->mgi($mgi) Returns : A string Args : None or a mgi locuslinkTitle : locuslink Usage : locuslink(); Function: Returns or stores a reference to an array containing locuslink data. Returns : An array reference Args : None or an array reference homolTitle : homol Usage : homol(); Function: Returns the homol entry associated with the object. Example : $homol = $unigene->homol or $unigene->homol($homol) Returns : A string Args : None or a homol entry restr_exprTitle : restr_expr Usage : restr_expr(); Function: Returns the restr_expr entry associated with the object. Example : $restr_expr = $unigene->restr_expr or $unigene->restr_expr($restr_expr) Returns : A string Args : None or a restr_expr entry gnm_terminusTitle : gnm_terminus Usage : gnm_terminus(); Function: Returns the gnm_terminus associated with the object. Example : $gnm_terminus = $unigene->gnm_terminus or $unigene->gnm_terminus($gnm_terminus) Returns : A string Args : None or a gnm_terminus scountTitle : scount Usage : scount(); Function: Returns the scount associated with the object. Example : $scount = $unigene->scount or $unigene->scount($scount) Returns : A string Args : None or a scount expressTitle : express Usage : express(); Function: Returns or stores a reference to an array containing tissue expression data Returns : An array reference Args : None or an array reference chromosomeTitle : chromosome Usage : chromosome(); Function: Returns or stores a reference to an array containing chromosome lines Returns : An array reference Args : None or an array reference stsTitle : sts Usage : sts(); Function: Returns or stores a reference to an array containing sts lines Returns : An array reference Args : None or an array reference txmapTitle : txmap Usage : txmap(); Function: Returns or stores a reference to an array containing txmap lines Returns : An array reference Args : None or an array reference protsimTitle : protsim Usage : protsim(); Function: Returns or stores a reference to an array containing protsim lines This should really only be used by ClusterIO, not directly Returns : An array reference Args : None or an array reference sequencesTitle : sequences Usage : sequences(); Function: Returns or stores a reference to an array containing sequence data. This is mostly reserved for ClusterIO parsers. You should use get_members() for get and add_member()/remove_members() for set. Returns : An array reference, or undef Args : None or an array reference or undef speciesTitle : species Usage : $obj->species($newval) Function: Get/set the species object for this Unigene cluster. Example : Returns : value of species (a L<Bio::Species> object) Args : on set, new value (a L<Bio::Species> object or the binomial name, or undef, optional) Bio::ClusterI methodsdisplay_idTitle : display_id Usage : Function: Get/set the display name or identifier for the cluster This is aliased to unigene_id(). Returns : a string Args : optional, on set the display ID ( a string) descriptionTitle : description Usage : Bio::ClusterI->description("POLYUBIQUITIN") Function: get/set for the consensus description of the cluster This is aliased to title(). Returns : the description string Args : Optional the description string sizeTitle : size Usage : Bio::ClusterI->size(); Function: get for the size of the family, calculated from the number of members This is aliased to scount(). Returns : the size of the cluster Args : cluster_scoreTitle : cluster_score Usage : $cluster ->cluster_score(100); Function: get/set for cluster_score which represent the score in which the clustering algorithm assigns to this cluster. For UniGene clusters, there really is no cluster score that would come with the data. However, we provide an implementation here so that you can score UniGene clusters if you want to. Returns : a number Args : optionally, on set a number get_membersTitle : get_members Usage : Bio::ClusterI->get_members(($seq1, $seq2)); Function: retrieve the members of the family by some criteria Will return all members if no criteria are provided. At this time this implementation does not support specifying criteria and will always return all members. Returns : the array of members Args : Annotatable view at the object propertiesannotationTitle : annotation Usage : $obj->annotation($newval) Function: Get/set the L<Bio::AnnotationCollectionI> object for this UniGene cluster. Many attributes of this class are actually stored within the annotation collection object as L<Bio::AnnotationI> compliant objects, so you can conveniently access them through the same interface as you would e.g. access L<Bio::SeqI> annotation properties. If you call this method in set mode and replace the annotation collection with another one you should know exactly what you are doing. Example : Returns : a L<Bio::AnnotationCollectionI> compliant object Args : on set, new value (a L<Bio::AnnotationCollectionI> compliant object or undef, optional) Implementation specific methodsThese are mostly for adding/removing to array properties, and for methods with special functionality. add_memberTitle : add_member Usage : Function: Adds a member object to the list of members. Example : Returns : TRUE if the new member was successfully added, and FALSE otherwise. Args : The member to add. remove_membersTitle : remove_members Usage : Function: Remove the list of members for this cluster such that the member list is undefined afterwards (as opposed to zero members). Example : Returns : the previous list of members Args : none next_locuslinkTitle : next_locuslink Usage : next_locuslink(); Function: Returns the next locuslink from an array referred to using $obj->{'locuslink'} If you call this iterator again after it returned undef, it will re-cycle through the list of elements. Changes in the underlying array property while you loop over this iterator will not be reflected until you exhaust the iterator. Example : while ( my $locuslink = $in->next_locuslink() ) { print "$locuslink\n"; } Returns : String Args : None next_expressTitle : next_express Usage : next_express(); Function: Returns the next tissue from an array referred to using $obj->{'express'} If you call this iterator again after it returned undef, it will re-cycle through the list of elements. Changes in the underlying array property while you loop over this iterator will not be reflected until you exhaust the iterator. Example : while ( my $express = $in->next_express() ) { print "$express\n"; } Returns : String Args : None next_chromosomeTitle : next_chromosome Usage : next_chromosome(); Function: Returns the next chromosome line from an array referred to using $obj->{'chromosome'} If you call this iterator again after it returned undef, it will re-cycle through the list of elements. Changes in the underlying array property while you loop over this iterator will not be reflected until you exhaust the iterator. Example : while ( my $chromosome = $in->next_chromosome() ) { print "$chromosome\n"; } Returns : String Args : None next_protsimTitle : next_protsim Usage : next_protsim(); Function: Returns the next protsim line from an array referred to using $obj->{'protsim'} If you call this iterator again after it returned undef, it will re-cycle through the list of elements. Changes in the underlying array property while you loop over this iterator will not be reflected until you exhaust the iterator. Example : while ( my $protsim = $in->next_protsim() ) { print "$protsim\n"; } Returns : String Args : None next_stsTitle : next_sts Usage : next_sts(); Function: Returns the next sts line from an array referred to using $obj->{'sts'} If you call this iterator again after it returned undef, it will re-cycle through the list of elements. Changes in the underlying array property while you loop over this iterator will not be reflected until you exhaust the iterator. Example : while ( my $sts = $in->next_sts() ) { print "$sts\n"; } Returns : String Args : None next_txmapTitle : next_txmap Usage : next_txmap(); Function: Returns the next txmap line from an array referred to using $obj->{'txmap'} If you call this iterator again after it returned undef, it will re-cycle through the list of elements. Changes in the underlying array property while you loop over this iterator will not be reflected until you exhaust the iterator. Example : while ( my $tsmap = $in->next_txmap() ) { print "$txmap\n"; } Returns : String Args : None Bio::IdentifiableI methodsobject_idTitle : object_id Usage : $string = $obj->object_id() Function: a string which represents the stable primary identifier in this namespace of this object. For DNA sequences this is its accession_number, similarly for protein sequences This is aliased to unigene_id(). Returns : A scalar versionTitle : version Usage : $version = $obj->version() Function: a number which differentiates between versions of the same object. Higher numbers are considered to be later and more relevant, but a single object described the same identifier should represent the same concept Unigene clusters usually won't have a version, so this will be mostly undefined. Returns : A number Args : on set, new value (a scalar or undef, optional) authorityTitle : authority Usage : $authority = $obj->authority() Function: a string which represents the organisation which granted the namespace, written as the DNS name for organisation (eg, wormbase.org) Returns : A scalar Args : on set, new value (a scalar or undef, optional) namespaceTitle : namespace Usage : $string = $obj->namespace() Function: A string representing the name space this identifier is valid in, often the database name or the name describing the collection Returns : A scalar Args : on set, new value (a scalar or undef, optional) Bio::DescribableI methodsdisplay_nameTitle : display_name Usage : $string = $obj->display_name() Function: A string which is what should be displayed to the user the string should have no spaces (ideally, though a cautious user of this interface would not assume this) and should be less than thirty characters (though again, double checking this is a good idea) This is aliased to unigene_id(). Returns : A scalar Status : Virtual description()Title : description Usage : $string = $obj->description() Function: A text string suitable for displaying to the user a description. This string is likely to have spaces, but should not have any newlines or formatting - just plain text. The string should not be greater than 255 characters and clients can feel justified at truncating strings at 255 characters for the purposes of display This is already demanded by Bio::ClusterI and hence is present anyway. Returns : A scalar Bio::Factory::SequenceStreamI methodsnext_seqTitle : next_seq Usage : next_seq(); Function: Returns the next seq as a Seq object as defined by $seq->sequence_factory(), at present an empty Bio::Seq::RichSeq object with just the accession_number() and pid() set This iterator will not exhaust the array of member sequences. If you call next_seq() again after it returned undef, it will re-cycle through the list of member sequences. Example : while ( my $sequence = $in->next_seq() ) { print $sequence->accession_number() . "\n"; } Returns : Bio::PrimarySeqI object Args : None sequence_factoryTitle : sequence_factory Usage : $seqio->sequence_factory($seqfactory) Function: Get/Set the Bio::Factory::SequenceFactoryI Returns : Bio::Factory::SequenceFactoryI Args : [optional] Bio::Factory::SequenceFactoryI Private methods_annotation_valueTitle : _annotation_value Usage : Function: Private method. Example : Returns : the value (a string) Args : annotation key (a string) on set, annotation value (a string) _annotation_value_aryTitle : _annotation_value_ary Usage : Function: Private method. Example : Returns : reference to the array of values Args : annotation key (a string) on set, reference to an array holding the values _annotation_dblinkTitle : _annotation_dblink Usage : Function: Private method. Example : Returns : array of accessions for the given database (namespace) Args : annotation key (a string) dbname (a string) (optional on get, mandatory on set) on set, accession or ID (a string), and version _remove_dblinkTitle : _remove_dblink Usage : Function: Private method. Example : Returns : array of accessions for the given database (namespace) Args : annotation key (a string) dbname (a string) (optional)
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