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NAMEBio::Search::HSP::ModelHSP - A HSP object for model-based searchesSYNOPSISuse Bio::Search::HSP::ModelHSP; # us it just like a Bio::Search::HSP::ModelHSP object DESCRIPTIONThis object is a specialization of Bio::Search::HSP::ModelHSP and is used for searches which involve a query model, such as a Hidden Markov Model (HMM), covariance model (CM), descriptor, or anything else besides a sequence. Note that results from any HSPI class methods which rely on the query being a sequence are unreliable and have thus been overridden with warnings indicating they have not been implemented at this time.FEEDBACKMailing ListsUser feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists SupportPlease direct usage questions or support issues to the mailing list:bioperl-l@bioperl.org rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. Reporting BugsReport bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:https://github.com/bioperl/bioperl-live/issues AUTHOR - Chris FieldsEmail cjfields at bioperl dot orgAPPENDIXThe rest of the documentation details each of the object methods. Internal methods are usually preceded with a _newTitle : new Usage : my $obj = Bio::Search::HSP::ModelHSP->new(); Function: Builds a new Bio::Search::HSP::ModelHSP object Returns : Bio::Search::HSP::ModelHSP Args : Plus Bio::Seach::HSP::ModelHSP methods -algorithm => algorithm used (Infernal, RNAMotif, ERPIN, etc) -evalue => evalue -pvalue => pvalue -bits => bit value for HSP -score => score value for HSP (typically z-score but depends on analysis) -hsp_length=> Length of the HSP (including gaps) -identical => # of residues that that matched identically -conserved => # of residues that matched conservatively (only protein comparisons; conserved == identical in nucleotide comparisons) -hsp_gaps => # of gaps in the HSP -query_gaps => # of gaps in the query in the alignment -hit_gaps => # of gaps in the subject in the alignment -query_name => HSP Query sequence name (if available) -query_start => HSP Query start (in original query sequence coords) -query_end => HSP Query end (in original query sequence coords) -hit_name => HSP Hit sequence name (if available) -hit_start => HSP Hit start (in original hit sequence coords) -hit_end => HSP Hit end (in original hit sequence coords) -hit_length => total length of the hit sequence -query_length=> total length of the query sequence -query_seq => query sequence portion of the HSP -hit_seq => hit sequence portion of the HSP -homology_seq=> homology sequence for the HSP -hit_frame => hit frame (only if hit is translated protein) -query_frame => query frame (only if query is translated protein) -meta => optional meta data (sec structure, markup, etc) -custom_score=> custom score data metaTitle : meta Usage : my $meta = $hsp->meta(); Function: Returns meta data for this HSP or undef Returns : string of meta data or undef Args : [optional] string to set value Note : At some point very soon this will likely be a Bio::AnnotationI. Don't get used to a simple string! noncanonical_stringTitle : noncanonical_string Usage : my $nc_seq = $hsp->noncanonical_string(); Function: Returns noncanonical string (NC) data for this HSP or undef Returns : string of noncanonical data or undef Args : [optional] string to set value custom_scoreTitle : custom_score Usage : my $data = $hsp->custom_score(); Function: Returns custom_score data for this HSP, or undef Returns : custom_score data or undef Args : [optional] custom_score Note : This is a Get/Set used to deal with odd score-like data generated from RNAMotif (and other programs) where the score section can be customized to include non-standard data, including sequence data, user-based scores, and other values. Bio::Search::HSP::HSPI methodsImplementation of Bio::Search::HSP::HSPI methods followalgorithmTitle : algorithm Usage : my $r_type = $hsp->algorithm Function: Obtain the name of the algorithm used to obtain the HSP Returns : string (e.g., BLASTP) Args : [optional] scalar string to set value strandTitle : strand Usage : $hsp->strand('hit') Function: Retrieves the strand for the HSP component requested Returns : +1 or -1 (0 if unknown) Args : 'hit' or 'subject' or 'sbjct' to retrieve the strand of the subject. There is no strand available for 'query', as the query is a model and not a true sequence. seqUsage : $hsp->seq( [seq_type] ); Purpose : Get the query or sbjct sequence as a Bio::Seq.pm object. Example : $seqObj = $hsp->seq('sbjct'); Returns : Object reference for a Bio::Seq.pm object. Argument : seq_type = 'query' or 'hit' or 'sbjct' (default = 'sbjct'). : ('sbjct' is synonymous with 'hit') : default is 'sbjct' : Note: if there is no sequence available (eg for a model-based : search), this returns a LocatableSeq object w/o a sequence Throws : Propagates any exception that occurs during construction : of the Bio::Seq.pm object. Comments : The sequence is returned in an array of strings corresponding : to the strings in the original format of the Blast alignment. : (i.e., same spacing). See Also : seq_str(), Bio::Seq pvalueTitle : pvalue Usage : my $pvalue = $hsp->pvalue(); Function: Returns the P-value for this HSP or undef Returns : float or exponential (2e-10) P-value is not defined with NCBI Blast2 reports. Args : [optional] numeric to set value evalueTitle : evalue Usage : my $evalue = $hsp->evalue(); Function: Returns the e-value for this HSP Returns : float or exponential (2e-10) Args : [optional] numeric to set value gapsTitle : gaps Usage : my $gaps = $hsp->gaps( ['query'|'hit'|'total'] ); Function : Get the number of gaps in the query, hit, or total alignment. Returns : Integer, number of gaps or 0 if none Args : arg 1: 'query' = num gaps in query seq 'hit' = num gaps in hit seq 'total' = num gaps in whole alignment default = 'total' arg 2: [optional] integer gap value to set for the type requested query_stringTitle : query_string Usage : my $qseq = $hsp->query_string; Function: Retrieves the query sequence of this HSP as a string Returns : string Args : [optional] string to set for query sequence hit_stringTitle : hit_string Usage : my $hseq = $hsp->hit_string; Function: Retrieves the hit sequence of this HSP as a string Returns : string Args : [optional] string to set for hit sequence homology_stringTitle : homology_string Usage : my $homo_string = $hsp->homology_string; Function: Retrieves the homology sequence for this HSP as a string. : The homology sequence is the string of symbols in between the : query and hit sequences in the alignment indicating the degree : of conservation (e.g., identical, similar, not similar). Returns : string Args : [optional] string to set for homology sequence lengthTitle : length Usage : my $len = $hsp->length( ['query'|'hit'|'total'] ); Function : Returns the length of the query or hit in the alignment (without gaps) or the aggregate length of the HSP (including gaps; this may be greater than either hit or query ) Returns : integer Args : arg 1: 'query' = length of query seq (without gaps) 'hit' = length of hit seq (without gaps) 'total' = length of alignment (with gaps) default = 'total' arg 2: [optional] integer length value to set for specific type frameTitle : frame Usage : my ($qframe, $hframe) = $hsp->frame('list',$queryframe,$subjectframe) Function: Set the Frame for both query and subject and insure that they agree. This overrides the frame() method implementation in FeaturePair. Returns : array of query and subject frame if return type wants an array or query frame if defined or subject frame if not defined Args : 'hit' or 'subject' or 'sbjct' to retrieve the frame of the subject (default) 'query' to retrieve the query frame 'list' or 'array' to retrieve both query and hit frames together Note : Frames are stored in the GFF way (0-2) not 1-3 as they are in BLAST (negative frames are deduced by checking the strand of the query or hit) get_alnTitle : get_aln Usage : my $aln = $hsp->gel_aln Function: Returns a Bio::SimpleAlign representing the HSP alignment Returns : Bio::SimpleAlign Args : none Inherited from Bio::SeqFeature::SimilarityPairThese methods come from Bio::SeqFeature::SimilarityPairqueryTitle : query Usage : my $query = $hsp->query Function: Returns a SeqFeature representing the query in the HSP Returns : Bio::SeqFeature::Similarity Args : [optional] new value to set hitTitle : hit Usage : my $hit = $hsp->hit Function: Returns a SeqFeature representing the hit in the HSP Returns : Bio::SeqFeature::Similarity Args : [optional] new value to set significanceTitle : significance Usage : $evalue = $obj->significance(); $obj->significance($evalue); Function: Get/Set the significance value Returns : numeric Args : [optional] new value to set scoreTitle : score Usage : my $score = $hsp->score(); Function: Returns the score for this HSP or undef Returns : numeric Args : [optional] numeric to set value bitsTitle : bits Usage : my $bits = $hsp->bits(); Function: Returns the bit value for this HSP or undef Returns : numeric Args : none ModelHSP methods overridden in ModelHSPThe following methods have been overridden due to their current reliance on sequence-based queries. They may be implemented in future versions of this class.seq_indsfrac_identicalfrac_conservedmatchesnum_conservednum_identicalcigar_stringgenerate_cigar_stringpercent_identity
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