NAME

Bio::SearchIO::blast_pull - A parser for BLAST output

SYNOPSIS

    # do not use this class directly it is available through Bio::SearchIO
    use Bio::SearchIO;
    my $in = Bio::SearchIO->new(-format => 'blast_pull',
                               -file   => 't/data/new_blastn.txt');
    while (my $result = $in->next_result) {
        # this is a Bio::Search::Result::BlastPullResult object
        print "Results for ", $result->query_name(), "\n";
        while (my $hit = $result->next_hit) {
            print $hit->name(), "\n";
            while (my $hsp = $hit->next_hsp) {
                print "length is ", $hsp->length(), "\n";
            }
        }
    }

DESCRIPTION

This object implements a pull-parser for BLAST output. It is fast since it only does work on request (hence 'pull').

Currently only NCBI BLASTN and BLASTP are supported.

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.

  bioperl-l@bioperl.org                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Support

Please direct usage questions or support issues to the mailing list:

bioperl-l@bioperl.org

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:

  https://github.com/bioperl/bioperl-live/issues

AUTHOR - Sendu Bala

Email bix@sendu.me.uk

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

new

 Title   : new
 Usage   : my $obj = Bio::SearchIO::blast_pull->new();
 Function: Builds a new Bio::SearchIO::blast_pull object 
 Returns : Bio::SearchIO::blast_pull
 Args    : -fh/-file => BLAST output filename
           -format   => 'blast_pull'
           -evalue   => float or scientific notation number to be used
                        as an evalue cutoff for hits
           -score    => integer or scientific notation number to be used
                        as a score value cutoff for hits
           -piped_behaviour => 'temp_file'|'memory'|'sequential_read'

           -piped_behaviour defines what the parser should do if the input is
            an unseekable filehandle (eg. piped input), see
            Bio::PullParserI::chunk for details. Default is 'memory'.

next_result

 Title   : next_result
 Usage   : my $hit = $searchio->next_result;
 Function: Returns the next Result from a search
 Returns : Bio::Search::Result::ResultI object
 Args    : none

result_count

 Title   : result_count
 Usage   : my $count = $searchio->result_count
 Function: Returns the number of results we have processed.
 Returns : integer
 Args    : none

rewind

 Title   : rewind
 Usage   : $searchio->rewind;
 Function: Allow one to reset the Result iterator to the beginning, so that
           next_result() will subsequently return the first result and so on.

           NB: result objects are not cached, so you will get new result objects
           each time you rewind. Also, note that result_count() counts the
           number of times you have called next_result(), so will not be able
           tell you how many results there were in the file if you use rewind().

 Returns : n/a
 Args    : none