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NAMEBio::Tools::Run::Alignment::Gmap - Wrapper for running gmap.SYNOPSISuse Bio::Tools::Run::Alignment::Gmap; use Bio::SeqIO; my $sio = Bio::SeqIO->new(-file=>$filename ,-format=>'fasta'); my @seq; while(my $seq = $sio->next_seq()){ push @seq,$seq; } my $mapper =Bio::Tools::Run::Gmap->new(); my $result = $mapper->run(\@seq); DESCRIPTIONBioperl-run wrapper around gmap. See <http://www.gene.com/share/gmap/> for information about gmap.It requires a reference to an array of bioperl SeqI objects and returns a reference to a filehandle from which the gmap output can be read. One can explicitly set the name of the genome database (defaults to NHGD_R36) using the 'genome_db()' method. One can also explicitly set the flags that are passed to gmap (defaults to '-f 9 -5 -e') using the 'flags()' method. The name of the gmap executable can be overridden using the program_name() method and the directory in which to find that executable can be overridden using the program_dir() method. FEEDBACKMailing ListsUser feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists Reporting BugsReport bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:http://redmine.open-bio.org/projects/bioperl/ AUTHOR - George HartzellEmail hartzell@alerce.comDescribe contact details here CONTRIBUTORSAdditional contributors names and emails hereAPPENDIXThe rest of the documentation details each of the object methods. Internal methods are usually preceded with a _newTitle : new Usage : my $obj = new Bio::Tools::Run::Alignment::Gmap(); Function: Builds a new Bio::Tools::Run::Alignment::Gmap object Returns : an instance of Bio::Tools::Run::Alignment::Gmap Args : versionTitle : version Usage : print "gmap version: " . $mapper->version() . "\n"; Function: retrieves and returns the version of the gmap package. Example : Returns : scalar string containing the version number. Probably looks like YYYY-MM-DD. Args : none. program_nameTitle : program_name Usage : $mapper->program_name('gmap-dev'); my $pname = $mapper->program_name(); Function: sets/gets the name of the program to run. Returns : string representing the name of the executable. Args : [optional] string representing the name of the executable to set. program_dirTitle : program_dir Usage : $mapper->program_dir('/usr/local/sandbox/gmap/bin'); my $pdir = $mapper->program_dir(); Function: sets/gets the directory path in which to find the gmap executable. Returns : string representing the path to the directory. Args : [optional] string representing the directory path to set. input_fileTitle : input_file Usage : $mapper->input_file('/tmp/moose.fasta'); my $filename = $mapper->input_file(); Function: sets/gets the name of a file containing sequences to be mapped. Returns : string containing the name of the query sequence. Args : [optional] string representing the directory path to set. genome_dbTitle : genome_db Usage : $mapper->genome_db('NHGD_R36'); my $genome_db = $mapper->genome_db(); Function: sets/gets the name of the genome database, this will be passed to gmap using its '-d' flag. Returns : name of the genome database. Args : [optional] string representing the genome db to set. flagsTitle : flags Usage : $mapper->flags('-A -e -5'); my $flags = $mapper->flags(); Function: sets/gets the flags that will be passed to gmap. Returns : the current value of the flags that will be passed to gmap. Args : [optional] the flags to set. runTitle : run Usage : $mapper->run() Function: runs gmap Example : Returns : a file handle, opened for reading, for gmap's output. Args : An array of references query sequences (as Bio::Seq objects) _build_fasta_input_fileTitle : _build_fasta_input_file Usage : my $seq_file = $self->_build_fasta_input_file(@_); Function: Example : Returns : The name of the temporary file that contains the sequence. Args : A reference to an array of Bio::Seq objects.
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