|
NAMEBio::Tools::Run::Alignment::MAFFT - run the MAFFT alignment toolsSYNOPSIS# Build a MAFFT alignment factory $factory = Bio::Tools::Run::Alignment::MAFFT->new(@params); # Pass the factory a list of sequences to be aligned. $inputfilename = 't/cysprot.fa'; # $aln is a SimpleAlign object. $aln = $factory->align($inputfilename); # or where @seq_array is an array of Bio::Seq objects $seq_array_ref = \@seq_array; $aln = $factory->align($seq_array_ref); #There are various additional options available. DESCRIPTIONYou can get MAFFT from <http://mafft.cbrc.jp/alignment/software/>. "fftnsi" is the default method for Mafft version 4 in this implementation.See Bio::Tools::Run::Alignment::Clustalw for a description on how to specify parameters to the underlying alignment program. See the MAFFT manual page for a description of the MAFFT parameters. FEEDBACKMailing ListsUser feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated.bioperl-l@bioperl.org - General discussion http://bioperl.org/MailList.html - About the mailing lists SupportPlease direct usage questions or support issues to the mailing list:bioperl-l@bioperl.org rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. Reporting BugsReport bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:http://redmine.open-bio.org/projects/bioperl/ AUTHOR - Jason StajichEmail jason-at-bioperl.orgAPPENDIXThe rest of the documentation details each of the object methods. Internal methods are usually preceded with a _program_nameTitle : program_name Usage : $factory->program_name() Function: holds the program name Returns: string Args : None executableTitle : executable Usage : my $exe = $blastfactory->executable('blastall'); Function: Finds the full path to the 'codeml' executable Returns : string representing the full path to the exe Args : [optional] name of executable to set path to [optional] boolean flag whether or not warn when exe is not found program_pathTitle : program_path Usage : my $path = $factory->program_path(); Function: Builds path for executable Returns : string representing the full path to the exe Args : none program_dirTitle : program_dir Usage : $factory->program_dir(@params) Function: returns the program directory, obtained from ENV variable. Returns: string Args : error_stringTitle : error_string Usage : $obj->error_string($newval) Function: Where the output from the last analysis run is stored. Returns : value of error_string Args : newvalue (optional) versionTitle : version Usage : exit if $prog->version() < 1.8 Function: Determine the version number of the program Example : Returns : float or undef Args : none runTitle : run Usage : my $output = $application->run(\@seqs); Function: Generic run of an application Returns : Bio::SimpleAlign object Args : array ref of Bio::PrimarySeqI objects OR filename of sequences to run with alignTitle : align Usage : $inputfilename = 't/data/cysprot.fa'; $aln = $factory->align($inputfilename); or $seq_array_ref = \@seq_array; # @seq_array is an array of Seq objs $aln = $factory->align($seq_array_ref); Function: Perform a multiple sequence alignment Returns : Reference to a SimpleAlign object containing the sequence alignment. Args : Name of a file containing a set of unaligned fasta sequences or else an array of references to Bio::Seq objects. Throws an exception if argument is not either a string (eg a filename) or a reference to an array of Bio::Seq objects. If argument is string, throws exception if file corresponding to string name can not be found. If argument is Bio::Seq array, throws exception if less than two sequence objects are in array. _runTitle : _run Usage : Internal function, not to be called directly Function: makes actual system call to tcoffee program Example : Returns : nothing; tcoffee output is written to a temporary file OR specified output file Args : Name of a file containing a set of unaligned fasta sequences and hash of parameters to be passed to tcoffee _setinputTitle : _setinput Usage : Internal function, not to be called directly Function: Create input file for mafft programs Example : Returns : name of file containing mafft data input Args : Seq or Align object reference or input file name _setparamsTitle : _setparams Usage : Internal function, not to be called directly Function: Create parameter inputs for mafft program Example : Returns : parameter string to be passed to mafft program Args : name of calling object methodsTitle : methods Usage : my @methods = $self->methods() Function: Get/Set Alignment methods - NOT VALIDATED Returns : array of strings Args : arrayref of strings _version6Title : _version6 Usage : Internal function, not to be called directly Function: Check if the version of MAFFT is 6 Example : Returns : Boolean Args : None Bio::Tools::Run::BaseWrapper methodsno_param_checksTitle : no_param_checks Usage : $obj->no_param_checks($newval) Function: Boolean flag as to whether or not we should trust the sanity checks for parameter values Returns : value of no_param_checks Args : newvalue (optional) save_tempfilesTitle : save_tempfiles Usage : $obj->save_tempfiles($newval) Function: Returns : value of save_tempfiles Args : newvalue (optional) outfile_nameTitle : outfile_name Usage : my $outfile = $mafft->outfile_name(); Function: Get/Set the name of the output file for this run (if you wanted to do something special) Returns : string Args : [optional] string to set value to tempdirTitle : tempdir Usage : my $tmpdir = $self->tempdir(); Function: Retrieve a temporary directory name (which is created) Returns : string which is the name of the temporary directory Args : none cleanupTitle : cleanup Usage : $mafft->cleanup(); Function: Will cleanup the tempdir directory Returns : none Args : none ioTitle : io Usage : $obj->io($newval) Function: Gets a L<Bio::Root::IO> object Returns : L<Bio::Root::IO> Args : none
Visit the GSP FreeBSD Man Page Interface. |