NAME

Bio::Tools::Run::Phylo::Molphy::ProtML - A wrapper for the Molphy pkg app ProtML

SYNOPSIS

  use Bio::AlignIO;
  use Bio::TreeIO;
  use Bio::Tools::Run::Phylo::Molphy::ProtML;

  my %args = ( 'models' => 'jtt',
               'search' => 'quick',
               'other'  => [ '-information', '-w'] );
  my $verbose = 0; # change to 1 if you want some debugging output
  my $protml = Bio::Tools::Run::Phylo::Molphy::ProtML->new(-verbose => $verbose,
                                                          -flags   => \%args);

  die("cannot find the protml executable") unless $protml->executable;

  # read in a previously built protein alignment
  my $in = Bio::AlignIO->new(-format => 'clustalw',
                            -file   => 't/data/cel-cbr-fam.aln');
  my $aln = $in->next_aln;
  $protml->alignment($aln);

  my ($rc,$results) = $protml->run();

  # This may be a bit of overkill, but it is possible we could
  # have a bunch of results and $results is a
  # Bio::Tools::Phylo::Molphy object

  my $r = $results->next_result;
  # $r is a Bio::Tools::Phylo::Molphy::Result object

  my @trees;
  while( my $t = $r->next_tree ) {
      push @trees, $t;
  }

  print "search space is ", $r->search_space, "\n";
        "1st tree score is ", $tree[0]->score, "\n";

  my $out = Bio::TreeIO->new(-file => ">saved_MLtrees.tre",
                            -format => "newick");
  $out->write_tree($tree[0]);
  $out = undef;

DESCRIPTION

This is a wrapper for the exe from the Molphy (MOLecular PHYlogenetics) package by Jun Adachi & Masami Hasegawa. The software can be downloaded from <http://www.ism.ac.jp/ismlib/softother.e.html>. Note that PHYLIP (Joe Felsenstein) also provides a version of protml which this module is currently NOT prepared to handle. Use the package available directly from MOLPHY authors if you want to use the module in its present implementation (extensions are welcomed!).

The main components are the protml and nucml executables which are used to build maximum likelihood (ML) phylogenetic trees based on either protein or nucleotide sequences.

Here are the valid input parameters, we have added a longhand version of the parameters to help you understand what each one does. Either the longhand or the original Molphy parameter will work.

  Bioperl      Molphy           Description
  Longhand     parameter
  Model (one of these):
  ---------------
  jtt              j            Jones, Taylor & Thornton (1992)
  jtt-f            jf           JTT w/ frequencies
  dayhoff          d            Dahoff et al. (1978)
  dayhoff-f        d            dayhoff w/ frequencies
  mtrev24          m            mtREV24 Adachi & Hasegwa (1995)
  mtrev24-f        mf           mtREV24 w/ frequencies
  poisson          p            Poisson
  proportional     pf           Proportional
  rsr              r            Relative Substitution Rate
  rsr-f            rf           RSR w/ frequencies
  frequencies      f            data frequencies

  Search Strategy (one of these):
  ----------------
  usertrees        u            User trees (must also supply a tree)
  rearrangement    R            Local rearrangement
  lbp              RX           Local boostrap prob
  exhaustive       e            Exhaustive search
  star             s            Star decomposition search (may not be ML)
  quick            q            Quick Add OTU search (may not be ML)
  distance         D            ML Distance matrix --> NJDIST (need to supply
                                                               NJDIST tree)

  Others (can be some or all of these):
  ---------------
  norell-bp        b            No RELL-BP
  minimumevolution M            Minimum evolution

  sequential       S            Sequence is in Sequential format
                     _OR_
  interleaved      I            Sequence is in Interleaved format

  verbose          v            Verbose messages directed to STDERR
  information      i            Output some information (tree vals)
                   w            More some extra information (transition
                                                             matricies, etc)

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.

  bioperl-l@bioperl.org                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Support

Please direct usage questions or support issues to the mailing list:

bioperl-l@bioperl.org

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:

  http://redmine.open-bio.org/projects/bioperl/

AUTHOR - Jason Stajich

Email jason-AT-bioperl_DOT_org

CONTRIBUTORS

Additional contributors names and emails here

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

program_name

 Title   : program_name
 Usage   : >program_name()
 Function: holds the program name
 Returns:  string
 Args    : None

program_dir

 Title   : program_dir
 Usage   : ->program_dir()
 Function: returns the program directory, obtained from ENV variable.
 Returns:  string
 Args    :

new

 Title   : new
 Usage   : my $obj = Bio::Tools::Run::Phylo::Molphy::ProtML->new();
 Function: Builds a new Bio::Tools::Run::Phylo::Molphy::ProtML object
 Returns : Bio::Tools::Run::Phylo::Molphy::ProtML
 Args    : -alignment => the Bio::Align::AlignI object
           -save_tempfiles => boolean to save the generated tempfiles and
                              NOT cleanup after onesself (default FALSE)
           -tree => the Bio::Tree::TreeI object
           -params => a hashref of PAML parameters (all passed to
                                                    set_parameter)
           -executable => where the protml executable resides

See also: Bio::Tree::TreeI, Bio::Align::AlignI

run

 Title   : run
 Usage   : $protml->run();
 Function: run the protml analysis using the default or updated parameters
           the alignment parameter must have been set
 Returns : Bio::Tools::Phylo::Molphy
 Args    :

alignment

 Title   : alignment
 Usage   : $protml->align($aln);
 Function: Get/Set the Bio::Align::AlignI object
 Returns : Bio::Align::AlignI object
 Args    : [optional] Bio::Align::AlignI
 Comment : We could potentially add support for running directly on a file
           but we shall keep it simple
See also : L<Bio::SimpleAlign>, L<Bio::Align::AlignI>

tree

 Title   : tree
 Usage   : $protml->tree($tree, %params);
 Function: Get/Set the Bio::Tree::TreeI object
 Returns : Bio::Tree::TreeI
 Args    : [optional] $tree => Bio::Tree::TreeI,

 Comment : We could potentially add support for running directly on a file
           but we shall keep it simple
See also : L<Bio::Tree::Tree>

get_flags

 Title   : get_flags
 Usage   : my @params = $protml->get_flags();
 Function: returns the list of flags
 Returns : array of flag names coded in the way that
 Args    : none

set_flag

 Title   : set_flag
 Usage   : $protml->set_parameter($type,$val);
 Function: Sets a protml parameter, will be validated against
           the valid values as set in the %VALIDVALUES class variable.
           The checks can be ignored if one turns off param checks like this:
             $protml->no_param_checks(1)
 Returns : boolean if set was success, if verbose is set to -1
           then no warning will be reported
 Args    : $type => name of the parameter
           This can be one of 'search', 'model', 'other'
           $value => flag value
 See also: L<no_param_checks()>

get_parameters

 Title   : get_parameters
 Usage   : my %params = $protml->get_parameters();
 Function: returns the list of parameters as a hash
 Returns : associative array keyed on parameter names
 Args    : none

set_parameter

 Title   : set_parameter
 Usage   : $protml->set_parameter($param,$val);
 Function: Sets a protml parameter, will be validated against
           the valid values as set in the %VALIDVALUES class variable.
           The checks can be ignored if one turns off param checks like this:
             $protml->no_param_checks(1)
 Returns : boolean if set was success, if verbose is set to -1
           then no warning will be reported
 Args    : $param => name of the parameter
           $value => value to set the parameter to
 See also: L<no_param_checks()>

Bio::Tools::Run::WrapperBase methods

no_param_checks

 Title   : no_param_checks
 Usage   : $obj->no_param_checks($newval)
 Function: Boolean flag as to whether or not we should
           trust the sanity checks for parameter values
 Returns : value of no_param_checks
 Args    : newvalue (optional)

save_tempfiles

 Title   : save_tempfiles
 Usage   : $obj->save_tempfiles($newval)
 Function:
 Returns : value of save_tempfiles
 Args    : newvalue (optional)

outfile_name

 Title   : outfile_name
 Usage   : my $outfile = $protml->outfile_name();
 Function: Get/Set the name of the output file for this run
           (if you wanted to do something special)
 Returns : string
 Args    : [optional] string to set value to

tempdir

 Title   : tempdir
 Usage   : my $tmpdir = $self->tempdir();
 Function: Retrieve a temporary directory name (which is created)
 Returns : string which is the name of the temporary directory
 Args    : none

cleanup

 Title   : cleanup
 Usage   : $protml->cleanup();
 Function: Will cleanup the tempdir directory after a PAML run
 Returns : none
 Args    : none

io

 Title   : io
 Usage   : $obj->io($newval)
 Function:  Gets a L<Bio::Root::IO> object
 Returns : L<Bio::Root::IO>
 Args    : none